Publications
- 2007
- Lee, J., Bansal, T., Jayaraman, A., Bentley, W.E., and T.K. Wood (2007) Enterohemorrhagic Escherichia coli Biofilms are Inhibited by 7-Hydroxyindole and Stimulated by Isatin, Applied and Environmental Microbiology, (in press). Abstract
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Yung, C.W., Tullman, J.A., Payne, G.F., Bentley, W.E., and T.A. Barbari (2007) Transglutaminase Crosslinked Gelatin as a Tissue Engineering Scaffold, Journal of Biomedical Materials Research A, (Epub ahead of print). Abstract
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Li, J., Attila, C., Wang, L., Wood, T.K., Valdes, J.J., and W.E. Bentley (2007) Quorum Sensing in E. coli is Signaled by AI-2/LsrR: Effects on sRNA and Biofilm Architecture, Journal of Bacteriology, 73:4100-4109. Abstract
- Fernandes, R., Tsao, C.Y., Hashimoto, Y., Wang, L., Wood, T.K., Payne, G.F., and W.E. Bentley (2007) Magnetic Nanofactories: Localized Synthesis and Delivery of Quorum-Sensing Signaling Molecule Autoinducer-2 to Bacterial Cell Surfaces, Metabolic Engineering, 9:228-239.
Abstract
- Small, D.A., Chang, W., Bentley, W.E., and F. Toghrol (2007) Toxicogenomic Analysis of Sodium Hypochlorite Antimicrobial Mechanisms in Pseudomonas aeruginosa, Applied Microbiology and Biotechnology, 74:176-185.
Abstract
- Kim, E.J., Kramer, S.F., Hebert, C.G., Valdes, J.J., and W.E. Bentley (2007) Metabolic Engineering of the Baculovirus-Expression System via Inverse “Shotgun” Genomic Analysis & RNA Interference (dsRNA) Increases Product Yield and Cell Longevity, Biotechnology and Bioengineering, 98:645-654. Abstract
- March, J.C., and W.E. Bentley (2007) RNAi-based Tuning of Cell Cycling in Drosophila S2 cells- effects on Recombinant Protein Yield, Applied Microbiology and Biotechnology, 73: 1128-1135. Abstract
- Koev, S.T., Powers, M. A. Powers, Yi, H.M., Wu, L-Q., Bentley, W.E., Rubloff, G.W., Payne, G.F., and R. Ghodssi, (2007) Mechano-transduction of DNA Hybridization and Dopamine Oxidation through Electrodeposited Chitosan Network, Lab on a Chip, 7:103-111.
Abstract
- Small, D.A, Chang, W., Toghrol, F., and W.E. Bentley (2007) Comparative Global Transcription Analysis of Sodium Hypochlorite, Peracetic Acid, and Hydrogen Peroxide on Pseudomonas aeruginosa, Applied Microbiology and Biotechnology, 76:1093-1105. Abstract
- O’Connell, K.P., Kovaleva, E., Campbell, J.H., Anderson, P.E., Brown, S.G., Davis, D.C., Valdes, J.J., Welch, R.W., Bentley, W.E. and N.A. van Beek (2007) Production of a Recombinant Antibody Fragment in Whole Insect Larvae, Molecular Biotechnology, 36:44-51. Abstract
- Hung, C-W., Holoman, T.R.P., Kofinas, P., and W.E. Bentley (2007) Towards Oriented Assembly of Proteins onto Magnetic Nanoparticles, Biochemical Engineering Journal, (in press).
- Saini, S.S., Stanford, C., Lee, S.M., Park, J., DeShong, P., Bentley, W.E., and M. Dagenais (2007) Monolayer Detection of Biochemical Agents Using Etched-Core Fiber Bragg Grating Sensors, IEEE Photonics Technology Letters, 19:1341-1343. Abtract
- Lewandowski, A.T., Yi, H., Luo, X., Payne, G.F., Ghodssi, R., Rubloff, G.W., and W.E. Bentley (2007) Protein Assembly onto Patterned Microfabricated Devices through Enzymatic Activation of Fusion Pro-tag. Biotechnology and Bioengineering, (in press). Abstract
- March, J.C., and W.E. Bentley (2007) Methods for gene silencing with RNAi, Methods in Molecular Biology, 388:424-434. Abstract
- Hu, Y.C., and W.E. Bentley (2007) Alternative Bioreactor Strategy for Probing Infection and Production, Methods in Molecular Biology, 388:419-426. Abstract
- Cha, H.J., and W.E. Bentley (2007) Monitoring and Visualization of Baculovirus Infection Using Green Fluorescent Protein Strategy, Methods in Molecular Biology, 388:407-418. Abstract
-
2006
- Lewandowski, A.T., Small, D.A., Chen, T., Payne, G.F., and
W.E. Bentley (2006) Tyrosine-based “activatable pro-tag”:
enzyme-catalyzed protein capture and release, Biotechnology and
Bioengineering, 93:1207-1215.
- Chang, W., Small, D.A., Toghrol, F., and W.E. Bentley (2006)
Global Transcriptome Analysis of Staphylococcus aureus Response
to Hydrogen Peroxide, Journal of Bacteriology, 188:1648-1659.
- Gonzalez Barrios, A.F., Zuo R., Hashimoto, Y., Yang, L., Bentley
W.E., and T.K. Wood (2006) Autoinducer 2 Controls Biofilm Formation
in Escherichia coli Through a Novel Motility Quorum-sensing Regulator
(MqsR, B3022), Journal of Bacteriology, 188:205-316.
- Bleckwenn, N. A. and Bentley, W. E. (2006) Systems and Technology
Involving Bacteria, In CRC BME Handbook: Tissue Engineering and
Artificial Organs (JD Bronzino, ed.) CRC Press, Boca Raton, FL,
2.1-2.10.
- Yung, C., Barbari, T.A., and W.E. Bentley (2006) Integrated
Non-Invasive System for Quantifying Secreted Human Therapeutic
hIL2, Biotechnology and Bioengineering.
- Yang, L., Portugal, F.H., and W.E. Bentley (2006) Conditioned
Medium from Listeria innocua Stimulate Emergence from a Resting
State not in Response to Quorum Sensing Autoinducer AI-2, Biotechnology
Progress, 22:387-393.
- March, J.C., and W.E. Bentley (2006) Engineering Eukaryotic
Signal Transduction with RNAi: Enhancing Drosophila S2 Cell Growth
and Recombinant Protein Synthesis via Silencing of TSC1. Biotechnology
and Bioengineering.
- Park, J.J., Valentine, T.M., Yi, H., Payne, G.F., Bentley,
W.E., Ghodssi, R., and Gary W. Rubloff (2006) In situ Biomolecule
Assembly in Completely Packaged Microfluidic Devices, Lab on a
Chip.
- Yung, C.W., Barbari, T.A., and W.E. Bentley (2006) Counteracting
Apoptosis and Necrosis with Hypoxia Responsive Expression of Bcl-2D,
Metabolic Engineering.
- Cresce, A.V., Silverstein, J.S., Bentley, W.E., and P. Kofinas
(2006) Nanopatterning of Recombinant Proteins using Block Copolymer
Templates, Macromolecules, 39, 5826-5829.
- Dalal, N.G., Cha, H.J., Kramer, S.F., Kostov, Y., Rao, G. and
W.E. Bentley (2006) Rapid Non-invasive Monitoring of Baculovirus
Infection for Insect Larvae Using Green Fluorescent Protein Reporter
Under Early-to-Late Promoter and a GFP-Specific Optical Probe,
Process Biochemistry, 41:947-950.
- Li, J., Wang, L., Hashimoto, Y., Tsao, C-Y., Wood, T.K., Valdes,
J.J., Zafiriou, E., and W.E. Bentley (2006) Stochastic Model of
E. coli AI-2 Quorum Signal Circuit Reveals Alternative Synthesis
Pathways, Nature Molecular Systems Biology.
-
2005
- Wang, L., Hashimoto, Y., Tsao, C.-Y., Valdes, J.J., and W.E.
Bentley (2005) cAMP and cAMP Receptor Protein (CRP) Influence
Both Synthesis and Uptake of Extracellular Autoinducer-2 in Escherichia
coli, Journal of Bacteriology, 187:2066-76.
- Yi, H., Wu, L.Q., Rubloff, G.W., Ghodssi, R., Payne, G.F.,
and Bentley, W.E., (2005) Signal-Directed Sequential Assembly
of Biomolecules on Patterned Surfaces. Langmuir, 2:2104-2107.
- Tao, Y., Bentley, W.E., and T.K. Wood, (2005) Regiospecific
Oxidation of Naphthalene and Fluorene by Toluene Monooxygenase
and Engineered Toluene 4-Monooxygenase of Pseudomonas mendocina
KR1, Biotechnology and Bioengineering, 90:85-94.
- Cha, H.J., Shin, H.S., Lim, H.J., Cho, H.S., Dalal, N.N., Pham,
M.Q., and W.E. Bentley (2005) Comparative Production of Human
Interleukin-2 Fused with Green Fluorescent Protein in Several
Recombinant Expression Systems, Biochemical Engineering Journal,
24:225-233.
- Lu CH, Albano CR, Bentley WE, and G. Rao (2005) Quantitative
and kinetic study of oxidative stress Regulons using green fluorescent
protein, Biotechnology and Bioengineering, 89: 574-587.
- Bleckwenn, N. A., Bentley, W. E., Shiloach, J., (2005) Vaccinia
Virus-Based Expression of gp120 and EGFP: Survey of Mammalian
Host Cell Lines, Biotechnology Progress, 21:186-191.
- Bleckwenn, N. A., Bentley, W. E., Shiloach, J., (2005) Evaluation
of Production Parameters with the Vaccinia Virus Expression System
Using Microcarrier Attached HeLa Cells, Biotechnology Progress,
21:554-561.
- Bleckwenn, N. A., Bentley, W. E., Shiloach, J., (2005) Production
of Recombinant Protein Using the HeLa S3-Vaccinia Virus Expression
System: Bioreactor Perfusion and Effects of Post-Infection Temperature,
Journal of Bioscience, Biotechnology and Biochemistry, 69:1065-1072.
- Bleckwenn, N. A., Golding, H., Bentley, W. E., Shiloach, J.,
(2005) Production of Recombinant Proteins by Vaccinia Virus in
a Microcarrier Based Mammalian Cell Perfusion Bioreactor, Biotechnology
and Bioengineering, 90:663-674.
- Tao, Y, Bentley, W.E., and T.K. Wood (2005) Phenol and 2-Naphthol
Production by Toluene 4-Monooxygenases Using an Aqueous/Dioctyl
Phthalate system, Applied Microbiology & Biotechnology, 68:614-621.
- Dalal, N.G., Bentley, W.E., and H.J. Cha (2005) Facile Monitoring
of Baculovirus Infection for Foreign Protein Expression under
Very Late Polyhedrin Promoter using Green Fluorescent Protein
Reporter under Early-to-Lte Promoter, Biochemical Engineering
Journal, 24:27-30.
- Cha, H.J., Dalal, N.G., and W.E. Bentley (2005) Secretion of
Human Interleukin-2 Fused with Green Fluorescent Protein in Recombinant
Pichia pastoris, Applied Biochemistry and Biotechnology, 126:1-11.
- Powers, M.A., S. T. Koev, A. Schleunitz, H. Yi, V. Hodzic,
W. E. Bentley, G. F. Payne, G. W. Rubloff, and R. Ghodssi (2005)
Integrated Optical Sensing of Biofunctionalized Sites in BioMEMS,
Lab on a Chip, 5:583-586.
- Chryssis, A.N., Saini, S.S., Lee, S.M., Yi, H.M., Bentley,
W.E., and M. Dagenais (2005) Detecting Hybridization of DNA by
Highly Selective Evanescent Field Etched Core Fiber Bragg Grating
Sensors, IEEE Journal of Selected Topics in Quantum Electronics,
11:864-872.
- Chang, W., Small, D.A. Toghrol, F., and W.E. Bentley (2005)
Microarray Analysis of Toxicogenomic Effects of Peracetic Acid
on Pseudomonas aeruginosa, Environmental Science & Technology,
39:5893-5899.
- Chang, W., Small, D.A., Toghrol, F., and W.E. Bentley (2005)
Microarray Analysis of Pseudomonas aeruginosa Reveals Induction
of Pyocin Genes in Response to Hydrogen Peroxide, BMC Genomics,
6.115-129.
- Yi, H., Nisar, S., Lee, SY, Powers, MA, Bentley, WE., Payne,
GF, Ghodssi, R., Rubloff, GW, Harris, MT, Culver, JN (2005) Patterned
Assembly of Genetically Modified Viral Nanotemplates via Nucleic
Acid Hybridization, Nano Letters, 5:1031-1936.
- Wang, L., Li, J., March, J.C., Valdes, J.J., and W.E. Bentley
(2005), luxS-Dependent Gene Regulation in Escherichia coli K-12
Revealed by Genomic Expression Profiling, Journal of Bacteriology,
187:8350-8360.
- Yi, H., Wu, L.Q., Bentley, W.E., Ghodssi, R., Rubloff, G.W.,
Culver, J.N., Payne, G.F., (2005) Biofabrication with Chitosan,
Biomacromolecules, 6:2881-2894.
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2004
- March, J.C. and W.E. Bentley (2004) Insulin stimulates dsRNA
uptake in Drosophila S2 cells, BioTechniques, 37:898-890.
- Mohaddes-Ardabili, L., Martinez-Miranda, L.J., Salamanca-Riba,LG.,
Christou, A., Silverman, J., Bentley, W.E., and M. Al-Sheikhly(2004)
Preferred Orientation of DNA Oligonucleotide Probes on the (2x4)Reconstructed
Surface of (001) GaAs, Journal of Applied Physics, 95:6021-6024.
- Lu, C., Bentley, W.E., and G. Rao(2004) A High Throughput Approach
to Promoter Study Using Green Fluorescent Protein, Biotechnology
Progress, 20:1634-1640.
- Tao Y, Fishman A, Bentley WE, and T.K. Wood (2004) Altering
toluene 4-monooxygenase by active-site engineering for the synthesis
of 3-methoxycatechol, methoxyhydroquinone, and methylhydroquinone,
Journal of Bacteriology, 186:4705-13.
- March, J.C. and W.E. Bentley (2004) Quorum sensing and bacterial
cross-talk in biotechnology, Current Opinion in Biotechnology,
15(5):495-502.
- Fishman A, Tao Y, Bentley WE, and T.K. Wood (2004) Protein
engineering of toluene 4-monooxygenase of Pseudomonas mendocina
KR1 for synthesizing 4-nitrocatechol from nitrobenzene, Biotechnology
& Bioengineering, 87(6):779-90.
- Tao, Y., Fishman, A., Bentley, W.E., and T.K. Wood (2004) Oxidation
of Benzene to Catechol and 1,2,3-Trihydroxybenzene by Toluene
4-Monooxygenase of Pseudomonas mendocina KR1 and Toluene 3-Monooxygenase
of Ralstonia pickettii PKO1, Applied & Environmental Microbiology,
7:3814-3820.
- Cha, H.J., Dalal, N.G., and W.E. Bentley (2004) In vivo Monitoring
of Human Interleukin-2 using Green Fluorescent Protein Fusion
Partner in Pichia Pastoris, Biotechnology letters, 26:1157-1162.
- Yi, H., Wu, L.Q., Ghodssi, R., Rubloff, G.W., Payne, G.F.,
and W.E. Bentley (2004) A Robust Technique for Assembly of Nucleic
Acid Hybridization Chips Based on Electrochemically Templated
Chitosan, Analytical Chemistry, 76:365-372.
- Fernandes,R., Yi, H., Wu, L.Q., Rubloff, G.w., Ghodssi, R.,
Bentley, W.E., and G.F. payne (2004) Thermo-Biolithography: A
Technique for Patterning Nucleic Acids and Proteins, Langmuir,
20:906-913.
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2003
- Chen, T., Small, D.A., Wu, L.Q., McDermot, M.K., Bentley, W.E.,
and G.F. Payne (2003) Enzymatic Methods for in Situ Entrapment
and Cell Release, Biomacromolecules, 4:1558-1563.
- Kastantin MJ, Li S, Gadre AP, Wu LQ, Bentley, WE, Payne GF,
Rubloff GW, Ghodssi R (2003) Integrated fabrication of polymeric
devices for biological applications, Sensors and Materials, 15(6):295-311.
- Chen, T., Yi, H., Rubloff, G.W., Ghodssi, R., Bentley, W.E.,
and G.F. Payne (2003) Nature-inspired Creation of Protein-polysaccharide
Conjugate and its Subsequent Assembly onto a Patterned Surface,
Langmuir, 19:9382-9386.
- Lu, C., Bentley, W.E., and G. Rao (2003) Comparisons of Oxidative
Stress Respponse Genes in Aerobic Escherichia coli Fermentations,
Biotechnology and Bioengineering, 83:864-870.
- Mohaddes-Ardabili, L., Martinez-Miranda, L.J., Silverman, J.,
Christou, A., Salamanca-Riba, L.G., Bentley, W.E., Ohuchi, F.,
and M. Al-Sheikhly (2003) Attachment of DNA probes on gallium
arsenide surface, Applied Physics letters, 83:192-194.
- Fernandes, R., Wu, L.Q., Chen, T., Yi, H., Rubloff, G.W., Ghodssi,
R., Bentley, W.E., and G.F.Payne (2003) Electrochemically-Induced
Deposition of a Polysaccharide Hydrogel onto a Patterned Surface,
Langmuir, 19:4058-4062.
- Kramer, S.F., and W.E. Bentley (2003) RNA Interference as a
Metabolic Engineering Tool: Potential for in vivo Control of Protein
Expression in an Insect Larval Model, Metabolic Engineering, 5:183-190.
- Wu, C.F.,Bentley, W.E., Valdes, J.J., and J.W. Sekowski (2003)
DNA microarray for discrimination between pathogeic O157:H7 EDL933
and non-pathogenic Escherichia coli strains, Biosensors and Bioelectronics,
19:1-8.
- March, J.C., Rao, G., and W.E. Bentley (2003) Biotechnological
Applications of Green Florescent Protein, Applied Microbiology
and Biotechnology, 62:303-315.
- Bleckwenn, N.A., Bentley, W.E., and J. Shiloach (2003) Investigating
Protein Production Strategies with Recombinant Vaccinia virus,
Biotechnology Progress, 19:130-136.
- Hyunmin Yi, H., Wu, L.Q., Sumner, J.J., Gillespie, J.B., Payne,
G.F.,and W.E. Bentley (2003) Chiosan Scaffolds for Biomolecular
Assembly: Coupling Nucleic Acid Probes for Detecting Hybridization,
Biotechnology and Bioengineering, 83:646-652.
- Kramer, S.F., Kostov, Y., Rao, G., and W.E. Bentley (2003)
Ex Vivo Monitoring of Protein Production in Baculovirus-Infected
Trichoplusia ni Larvae with a GFP-Specific Optical Probe, Biotechnology
and Bioengineering, 83:241-247.
-
2002
- Lu, C., Albano, C.R., Bentley, W.E., and G. Rao (2002) Differential
Rates of Gene Expression Monitored by Green Fluorescent Protein,
Biotechnology and Bioengineering, 79:429-37.
- DeLisa, M.P., and W. E. Bentley (2002) Bacterial Autoinduction:
Looking Outside the Cell for New Metabolic Engineering Targets,
Microbial Cell Factories, 1:5-12.
- Wu, Li-Qun, Yi, H., Li, S., Rubloff, G.W., Bentley, W.E., Ghodssi,
R.,and G.F. Payne, (2002) Spatially-selective Deposition of a
Reactive Polysaccharide Layer onto a Patterned Template, Langmuir,
19:519-524.
- Wu, Li-Qun, Gadre, A.P, Yi, Hyunmin, Kastantin, M.J., Rubloff,
G.W., Bentley, W.E., Payne, G.F., and Reza Ghodssi, (2002) Voltage-dependent
Assembly of the Polysaccharide Chitosan onto an Electrode Surface,
Langmuir, 8:8620-8625.
- Li, J., Xu, H., Herber, W.K., Bentley, W.E., and G. Rao (2002)
Integrated Bioprocessing in Saccharomyces cerevisiae Using Green
Fluorescent Protein as a Fusion Partner, Biotechnology and Bioengineering,
79:682-693.
- Li, J., Xu, H., Bentley, W.E. and Rao, G. (2002) Impediments
to Secretion of Green Fluorescent Protein and its Fusion from
Saccharomyces cerevisiae, Biotechnology Progress, 18:831-838.
- Wu, C.F., Cha, H.J., Valdes, J.J., and W. E. Bentley (2001)
GFP-Immobilized Enzymes: Degradation of Paraoxon via Organophosphorous
Hydrolase in a Packed Column, Biotechnology and Bioengineering,
77:212-218.
- Cha, H.J., Dalal, N.G., Pham, M.-Q., Kramer, S.F., Vakharia,
V.N., and W.E. Bentley (2002) Monitoring Foreign Protein Expression
from Baculovirus p10 and polh Promoters in Insect Larvae, BioTechniques,
35:986-991 .
-
2001
- Wu, C.F., Valdes, J.J.., Rao, G., and W.E. Bentley (2001) Enhancement
of Organophosphorus Hydrolase Yield in E. coli Using Multiple
Gene Fusions, Biotechnology and Bioengineering, 75:100-103.
- Gotoh, T., Miyazaki, Y., Sato, W., Kikuchi, K.-I., and Bentley,
W. E.(2001) Proteolytic Activity and Recombinant Protein Production
of Virus-infected Sf-9 Insect Cell Culture Supplemented with Cysteine
Protease and Carboxyl Protease Inhibitors, Journal of Bioscience
and Bioengineering, 92:248-255 .
- Cho, H.S., Shin, H.S., Bentley, W.E., and H. J. Cha (2001)
Partial Recovery of Cell Membrane Bounded Human Interleukin-2
Fusion Protein from Insect Cell Debris by Using Various Detergent
Extractions, Biotechnology Letters, 23:1957-1961 .
- DeLisa, M.P., Gill, R.T., and W.E. Bentley (2001) Mapping Stresses
in Escherichia coli to Improve Yield, EFB Recombinant Protein
Production, Kluwer Academic Publishers, pp.43-54 .
- Hu, Y.-C., and W. E. Bentley (2001) The Effect of MOI Ratio
on the Composition and Yield of Chimeric Infectious Bursal Disease
Virus-like Particles by Baculovirus Co-infection: Deterministic
Predictions and Experimental Results, Biotechnology and Bioengineering,
75:104-119.
- Srivastava, R., Peterson, M.S., and W.E. Bentley (2001) Stochastic
Kinetic Analysis of the Escherichia coli Stress Circuit Using
s32 Targeted Antisense, Biotechnology and Bioengineering, 75:120-129.
- 78. Wu, C.F., Valdes, J.J. and W.E. Bentley (2001) Effects
of in situ Cobalt Ion Addition on the Activity of a GFP-OPH Fusion
Protein: The Fermentation Kinetics, Biotechnology Progress, 17:606-611.
- DeLisa, M.P., Chae, H.J., Rao, G., Weigand, W.A., Valdes, J.J.,
and W.E. Bentley (2001) Generic Model Control of Induced Protein
Expression in High Cell Density Cultivation of Escherichia coli
Using On-line GFP-Fusion Monitoring. Bioprocess and Biosystems
Engineering, 24:83-91.
- Lee, T.S., Chan, S.H., Weigand, W.A., and W.E. Bentley (2001)
A Metabolic Model for Thiodiglycol Degradation: Capacity Constraint
Leads to Byproduct Accumulation. Bioprocess and Biosystems Engineering,
24:33-38.
- Albano, C. R., Lu, Canghai, Bentley, W.E., and Govind Rao.
(2001) High Throughput Studies of Gene Expression Using Green
Fluorescent Protein-Oxidative Stress Promoter Probe Constructs:
The Potential for Living Chips. J. Biomolecular Screening. 6:421-428.
- DeLisa, M.P., Valdes, J.J., and W.E. Bentley (2001) Quorum
Signaling via AI-2 Communicates the “Metabolic Burden?Associated
with Heterologous Protein Expression in Escherichia coli. Biotechnology
and Bioengineering, 75:439-450.
- DeLisa, M.P., Wu, C.F., Wang, L., Valdes, J.J., and W.E. Bentley
(2001) DNA Microarray-Based Identification of Genes Controlled
by AI-2-stimulated Quorum Sensing in Escherichia coli. Journal
of Bacteriology. 183:5239-5247.
- Gotoh, T., Miyazaki, Y., Kikuchi, KI, and W.E. Bentley (2001)
Investigation of Sequential Behavior of Carboxyl Protease and
Cysteine Protease Activities in Virus-infected Sf-9 Insect Cell
Culture by Inhibition Assay, Applied Microbiology and Biotechnology
,56:742-749.
- Gill, R.T., DeLisa, M.P., Valdes, J.J., and W.E. Bentley (2001)
Genomic Analysis of High Cell Density Recombinant Escherichia
coli Fermentation and “Cell Conditioning?for Improved Recombinant
Protein Yield. Biotechnology and Bioengineering, 72: 85-95.
- DeLisa, M.P., Valdes, J.J., and W.E. Bentley (2001) Mapping
Stress-Induced Changes in Autoinducer AI-2 Production in Chemostat
Cultivated Escherichia coli K-12, Journal of Bacteriology, 183:2918-2928.
- Chen, T., Vazquez-Duhalt, R., Wu, C.F., Bentley, W.E., and
G.F. Payne (2001) Combinatorial Screening for Enzyme-mediated
Coupling. Tyrosinase-Catalyzed Coupling to Create Protein-Chitosan
Conjugates, Biomacromolecules. 2:456-452.
-
2000
- Cha, H.J., Wu, C.-F., Valdes, J.J., Rao, G., and W. E. Bentley
(2000) Observations of Green Fluorescent Protein as a Fusion Partner
in Genetically Engineered Escherichia coli: Monitoring Protein
Expression and Solubility, Biotechnology and Bioengineering, 67:565-574.
- Wu, C.-F., Cha, H.J., Rao, G., Valdes, J.J., and W. E. Bentley
(2000) A Green Fluorescent Protein Fusion Strategy for Monitoring
the Expression, Cellular Localization, and Separation of Biologically
Active Organophosphorous Hydrolase, Applied Microbiology and Biotechnology,
54:78-83.
- Hu, Y.C., and W. E. Bentley (2000) A Kinetic and Statistical
Thermodynamics-based Model for Baculovirus Infection and Virus-like
Particle Assembly, Chemical Engineering Science, 55:3991-4008.
- Chae, H.J., DeLisa, M.P., Cha, H.J., Weigand, W.A., Rao, G.,
and W.E. Bentley (2000) A Framework for On-line Optimization of
Recombinant Protein Expression in High Cell Density Escherichia
coli Cultures using GFP-Fusion Monitoring, Biotechnology and Bioengineering,
69: 275-285.
- Gill, R.T., Valdes, J.J., and W.E. Bentley (2000) A Comparative
Study of Global Stress Gene Regulation in Response to Overexpression
of Recombinant Proteins in Escherichia coli. Metabolic Engineering,
2: 178-189.
- DeLisa, M.P., Zhang, Z., Shiloach, M., Pilevar, S., Davis, C.C.,
Bentley, W.E., and J.S. Sirkis (2000) Evanescent Wave Long Period
Fiber Bragg Grating as an Immobilized Antibody Biosensor. Analytical
Chemistry, 72:2895-2900.
- Gill, R.T., Shiloach, M.S., DeLisa, M.P., Holoman, T.R., and
W.E. Bentley (2000) OmpT Expression and Activity Increase in Response
to Recombinant Protein Chloramphenicol Acetyltransferase Overexpression
and Heat Shock in E. coli. Journal of Microbiology and Molecular
Biotechnology. 2:283-289.
- Lee, T.-S. , Chan, S.H., Weigand, W.A., and W. E. Bentley (2000)
Biocatalytic Transformation of [(2-Hydroxyethyl)thio]acetic Acid
and Thiodiglycolic Acid from Thiodiglycol by Alcaligenes xylosoxydans
ssp. xylosoxydans (SH91), Biotechnology Progress, 16:363-367.
- Srivastava, R., Cha, H.J., Peterson, M.S., and W.E. Bentley
(2000) Antisense Downregulation of s32 as a Transient Metabolic
Controller in Escherichia coli: Effects on Yield of Active Organophosphorous
Hydrolase, Applied and Environmental Microbiology, 66: 4366-4371.
- Madurawe, R., Chase, T., Tsao, E.I., and W.E. Bentley (2000)
A Recombinant Lipoprotein Antigen Against Lyme Disease Expressed
in E. coli: Fermentor Operating Strategies for Improved Yield.
Biotechnology Progress, 16:571-576.
- Li, J., Wang, S., VanDeusen, W.J., Schultz, L.D., George, H.A.,
Herber, W.K., Chae, H.J., Bentley, W.E., Rao, G. (2000) Green
Fluorescent Protein in Saccharomyces cerevisiae: Real-Time Studies
of the GAL1 Promoter, Biotechnology and Bioengineering, 70:187-196.
-
1999
- Pham, M.Q., Naggie, S., Wier, M., Cha, H.J., and W.E. Bentley
(1999) Human Interleukin-2 Production in Insect (Trichoplusia
ni) Larvae: Effects and Partial Control of Proteolysis, Biotechnology
and Bioengineering, 62:175-182.
- Cha, H.J., Srivastava, R., Vakharia, V., Rao, G., and W. E.
Bentley (1999) Green Fluorescent Protein as a Non-invasive "Stress
Probe" in Resting Recombinant Escherichia coli Culture, Applied
and Environmental Microbiology, 65:409-414.
- Cha, H.J., Dalal, N.G., Vakharia, V.N., and W. E. Bentley (1999)
Expression and Purification of Human Interleukin-2 as a Fusion
with Green Fluorescent Protein in Suspended Sf-9 Insect Cells,
Journal of Biotechnology, 69:9-17.
- Cha, H.J., Dalal, N.G., Pham, M.Q., and W.E. Bentley (1999)
Purification of Human Interleukin-2 Fusion Protein Produced in
Insect Larvae is Facilitated by Fusion with Green Fluorescent
Protein and Metal Affinity Ligand, Biotechnology Progress, 15(2),
283-286.
- Wang, M.Y., Wang. S.L., Bentley, W.E., and G.C. Liu (1999)
Using Glucose Feed to Regulate pH Values of Culture Media and
Increase the Production of Baculovirus (BmNPV) and Foreign Protein
(HBsAg), Process Biochemistry, 34:295-301.
- Ramirez, D.L. and W.E. Bentley (1999) Modeling Inducer and
Amino Acid Fed-batch Cultures: Characterization of Cell Stress
and Protein Turnover, Journal of Biotechnology, 71:39-58.
- Hu, Y.-C., Vakharia, V., Edwards, G., and W. E. Bentley (1999)
Chimeric Infectious Bursal Disease Virus-like Particles Expressed
in Insect Cells and Purified by Immobilized Metal Affinity Chromatography,
Biotechnology and Bioengineering, 63:721-729.
- Dalal, N.G., and W. E. Bentley (1999) Mathematical Characterization
of Insect Cell (Sf-9) Death in Suspended Culture, Biotechnology
Letters, 21: 325-329.
- Harcum, S.W., and W. E. Bentley (1999) The Heat-shock and Stringent
Responses have Overlapping Protease Activity in E. coli: Implications
for Heterologous Protein Yield, Applied Biochemistry and Biotechnology,
80:1-15.
- DeLisa, M.P., Li, J.C., Rao, G., Weigand, W.A., and W.E. Bentley
(1999) Monitoring GFP-operon Fusion Protein Expression During
High Cell Density Cultivation of Escherichia coli Using an On-line
Optical Sensor, Biotechnology and Bioengineering, 65:54-64.
- Gill, R.T., Valdes, J.J., and W.E. Bentley (1999) RTPCR Differential
Display Analysis of Escherichia coli Global Gene Regulation in
Response to Heat Shock, Applied and Environmental Microbiology,
65:5386-5393.
- Hu, Y.-C. and W.E. Bentley (1999) Enhancing Yield of Infectious
Bursal Disease Virus Structural Proteins in Baculovirus Expression
Systems: Focus on Media, Protease Inhibitors, and Dissolved Oxygen,
Biotechnology Progress, 15:1065-1071.
- Cha, H.J, Dalal, N.G., Pham, M.Q., Vakharia, V.N., Rao, G.,
and W. E. Bentley (1999) Insect Larval Expression Process is Optimized
by Generating Fusions with Green Fluorescent Protein, Biotechnology
and Bioengineering, 65:316-324.
-
1998
- Gill, R.T., Cha, H.J., Jain, A., Rao, G., and W.E. Bentley
(1998) Generating Controlled Reducing Environments in Aerobic
Recombinant E. coli Fermentations: Effect on Cell Growth, Oxygen
Uptake, and in vivo CAT Activity, Biotechnology and Bioengineering,
59:248-259.
- Albano, R., Randers-Eichhorn, L., Bentley, W.E., and G. Rao
(1998) Green Fluorescent Protein as a Real Time Quantitative Reporter
of Heterologous Protein Production, Biotechnology Progress, 14:
351-354.
- Naggie, S. and W.E. Bentley (1998) Appearance of Protease Activities
Coincides with p10 and polyhedrin-driven Protein Production in
Baculovirus Expression System: Effects on Yield, Biotechnology
Progress, 14:227-232.
- Gotoh, T., Iwanaga, T., Kikuchi, K, and Bentley, WE (1998)
A Novel Support for Membrane Enzyme Immobilization: Gel Beads
Containing Polymerized Phospholipid Vesicles, Biotechnology and
Applied Biochemistry, 27: 197-204.
- Bentley, W.E., Madurawe, R.D., Gill, R.T., Shiloach, M., Chase,
T.E., Pulliam-Holoman, T.R., and J.J. Valdes (1998) Generation
of a Histidine-Tagged Antibotulinum Toxin Antibody Fragment in
E. coli: Effects of Post-induction Temperature on Yield and IMAC
Binding-Affinity, Journal of Industrial Microbiology and Biotechnology,
21:275-282.
-
1997
- Pulliam, T. R., Winston, S., and W.E. Bentley (1997) Tryptophan
Regulated Expression and Aqueous Two-Phase Separation of Recombinant
HIV-Fusion Peptides, Enzyme and Microbial Technology, 20: 47-51.
- Lee, T.-S., Weigand, W.A., and W. E. Bentley (1997) Observations
of Metabolite Formation and Variable Yield in Thiodiglycol Biodegradation
Processes: Impact on Reactor Design, Applied Biochemistry and
Biotechnology, 63/65: 743-757.
- Hu, Y.C., Wang, M.Y., and W.E. Bentley (1997) A Tubular Bioreactor
for the Infection of Insect Cells with Recombinant Baculovirus,
Cytotechnology, 24: 143-152.
- Randers-Eichhorn, L., Albano, C.R., Sipior, J., Bentley, W.E.,
and G. Rao (1997) An On-line Green Fluorescent Protein Sensor
with LED Excitation, Biotechnology and Bioengineering, 55: 921-927.
- Naggie, S., Hu, Y.C., Pulliam-Holoman, T.R., and W.E. Bentley
(1997) Substrate (Gelatin) Gel Electrophoretic Method for Analysis
of Protease Activity in Insect (Sf-9) Cells, Biotechnology Techniques,
11: 297-301.
- Pilon, A., Yost, P., Chase, T. Lohnas, and W.E. Bentley (1997)
Ubiquitin Fusion Technology: Bioprocessing of Peptides, Biotechnology
Progress, 13:374-379.
- Cha, H.J., Pham, M.Q., Rao, G., and W. E. Bentley (1997) Expression
of Green Fluorescent Protein in Insect Larvae and its Application
for Heterologous Protein Production, Biotechnology and Bioengineering,
56:238-243.
- Cha, H.J., Choi, S.S., Yoo, Y.J., and W.E. Bentley (1997) Enhancement
of Production of Cloned Glucoamylase under Low Aeration Condition
from Recombinant Yeast Using a SUC2 Promoter, Process Biochemistry,
32, 679-684.
- Cha, H.J., Gotoh, T., and W. E. Bentley (1997) Simplification
of Titer Determination for Recombinant Baculovirus by Green Fluorescent
Protein Marker, BioTechniques, 23: 782-786.
-
1996
- Pham, M.Q., Harvey, S.P., Weigand, W.A., and W.E. Bentley (1996)
Reactor Comparisons for the Biodegradation of Thiodiglycol, a
Product of Mustard Gas Hydrolysis, Applied Biochemistry and Biotechnology,
57/58: 779-789.
- Wang, M.Y., Pulliam, T.R., Valle, M., Vakharia, V. and W.E.
Bentley (1996) Kinetic Analysis of Alkaline Protease Activity,
Recombinant Protein Production and Metabolites for Infected Insect
(Sf-9) Cells under Different DO Levels, Journal of Biotechnology,
46: 243-254.
- Pilon, A., Yost, P., Chase, T. Lohnas, and W.E. Bentley (1996)
High-level Expression and Efficient Recovery of Ubiquitin Fusion
Proteins from Escherichia coli, Biotechnology Progress, 12:331-337.
- Lee, T.S., Pham, M.Q., Weigand, W.A., Harvey, S.P., and W.E.
Bentley (1996) Bioreactor Strategies for the Treatment of Growth-Inhibitory
Waste: An Analysis of Thiodiglycol Degradation, the Main Hydrolysis
Product of Sulfur Mustard, Biotechnology Progress, 12:533-539.
- Albano, R, Randers-Eichhorn, L., Bentley, W.E., and G. Rao
(1996) Quantitative Measurement of Green Fluorescent Protein Expression
and Chromophore Cyclization, Biotechnology Techniques, 10: 953-958.
-
1995
- Chen, Q., Bentley, W.E. and W. A. Weigand (1995), Optimization
for a Recombinant E. coli Fed-Batch Fermentation, Applied Biochemistry
and Biotechnology, 51/52: 449-461.
- Ko, Y.-F., Bentley, W.E., and W. A. Weigand (1995), The Effect
of Cellular Energetics on Foreign Protein Production, Applied
Biochemistry and Biotechnology, 50:145-159.
- Ramirez, D. M. and W. E. Bentley (1995), Fed-Batch Feeding
and Induction Policies that Improve Foreign Protein Synthesis
and Stability by Avoiding Stress Responses, Biotechnology and
Bioengineering, 47:596-608.
-
1994
- Ko, Y.-F., Bentley, W. E. and W. A. Weigand (1994), A Metabolic
Model of Cellular Energetics and Carbon Flux During Aerobic E.
coli Fermentation, Biotechnology and Bioengineering, 43:847-855
.
- Wang, M.-Y., Bentley, W. E. and V. Vakharia (1994), Purification
of a Recombinant Protein Produced in a Baculovirus Expression
System by Immobilized Metal Affinity Chromatography, Biotechnology
and Bioengineering 43: 349-356.
- Wang, M.-Y. and W. E. Bentley (1994), Continuous Insect Cell
(Sf-9) Culture with Aeration through Sparging, Applied Microbiology
and Biotechnology 41:317-323.
- Bentley, W. E., Wang, M.-Y. and V. Vakharia (1994), Development
of an Efficient Bioprocess for Poultry Vaccines Using High Density
Insect Cell Culture, Annals New York Academy of Sciences 745:336-359.
- Bentley, W.E. (1994), Undergraduate Researchers within a Biochemical
Engineering Research Program: Factors Influencing Research Quality
and Learning Experience, Annual Conference Proceedings, American
Society for Engineering Education, 1994(1):1461-1464.
- Bentley, W.E., Kebede B., Franey, T., and M.Y. Wang (1994),
Segregated Characterization of Recombinant Epoxide Hydrolase Synthesis
via the Baculovirus / Insect Cell Expression System, Chemical
Engineering Science. 49:4133-4141.
- Pulliam, T. R., Winston, S. and W.E. Bentley (1994), Expression
and Aqueous Two-Phase Separation of HIV582-b-gal Fusion Protein,
Journal of Long-Term Effects of Medical Implants. 4(1): 56.
-
1993
- Ramirez, D. M. and W. E. Bentley (1993), Enhancement of Recombinant
Protein Synthesis and Stability via Coordinated Amino Acid Addition,
Biotechnology and Bioengineering, 41:557-565.
- Wang, M.-Y., Kwong, S., and W. E. Bentley (1993), Effects of
Oxygen/Glucose/Glutamine Feeding on Insect Cell Baculovirus Expression:
A Study of Epoxide Hydrolase Production, Biotechnology Progress,
9:355-361.
- Wang, M.-Y., Vakharia, V., and W. E. Bentley (1993), Expression
of Epoxide Hydrolase in Insect Cells: A Focus on the Infected
Cell, Biotechnology and Bioengineering, 42:240-246.
- Bentley, W. E. and O. E. Quiroga (1993), Investigations of
Subpopulation Heterogeneity and Plasmid Stability in Recombinant
E. coli via a Simple Segregated Model, Biotechnology and Bioengineering,
42:222-234.
- Ko, Y.-F., Bentley, W. E. and W. A. Weigand (1993), An Integrated
Metabolic Modeling Approach to Describe the Energy Efficiency
of E. coli Under Oxygen-Limited Conditions: Cellular Energetics
and Carbon Flux, Biotechnology and Bioengineering, 42:843-853.
- Harcum, S. W. and W. E. Bentley (1993), Detection, Quantification,
and Characterization of Proteases in Recombinant Escherichia coli,
Biotechnology Techniques, 7:441-447.
- Harcum, S. W. and W. E. Bentley (1993), Response Dynamics of
26, 34, 39, 54, and 80 kDa Proteases in Induced Cultures of Recombinant
Escherichia coli, Biotechnology and Bioengineering, 42:675-685.
- Wang, M.-Y., Wester, K. and W. E. Bentley (1993), Glutamine
Determination in Insect Cell Culture Media, Biotechnology Techniques,
7:841-846.
-
1992
- Harcum, S. W., Ramirez, D. M. and W. E. Bentley (1992), Optimal
Nutrient Feed Policies for Heterologous Protein Production, Applied
Biochemistry and Biotechnology, 34/35:161-173.
- Bentley, W. E., Davis, R. H. and D. S. Kompala (1991), Dynamics
of Induced CAT Expression in E. coli, Biotechnology and Bioengineering,
38:749-760.
- Bentley, W. E. and D. S. Kompala (1990), Stability in Recombinant
Continuous Cultures - A Metabolic Approach, Biotechnology Letters,
12(5):329-334.
- Bentley, W. E. and D. S. Kompala (1990), Plasmid Instability
in Batch Cultures of Recombinant Bacteria: A Laboratory Experiment,
Chemical Engineering Education, 24(3):168-172.
- Bentley, W. E. and D. S. Kompala (1990), Optimal Induction of
Protein Expression in Recombinant Bacterial Cultures, in Biochemical
Engineering VI, Annals New York Academy of Sciences, 589:120-138.
- Bentley, W.E., Mirjalili, N., Andersen, D. C., Kompala, D. S.
and R. H. Davis (1990), Plasmid Encoded Protein: The Principal Factor
in the "Metabolic Burden" Associated with Recombinant
Bacteria, Biotechnology and Bioengineering, 35(7):668-681.
- Bentley, W. E. and D. S. Kompala (1989), A Novel Structured Kinetic
Modeling Approach for the Analysis of Plasmid Instability in Recombinant
Bacterial Cultures, Biotechnology and Bioengineering, 33(1): 49-61.
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